diff --git a/LifeSciences/gatk-4.0.0.0/build.sh b/LifeSciences/gatk-4.0.0.0/build.sh
new file mode 100644
index 0000000000000000000000000000000000000000..8e280c9f6d0a3a6d14b64e58d8f74fbe0520c1dc
--- /dev/null
+++ b/LifeSciences/gatk-4.0.0.0/build.sh
@@ -0,0 +1,71 @@
+#!/bin/bash
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash build.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+create_dir()
+{
+    local path=$1
+    [[ "$path" =~ .*/$ ]] && path=${path%/*}
+    if [[ ! -d "$path" && ! -f "$path" ]]; then mkdir -p $path; else path=$path`date "+%y%m%d%H%M%S"` && mkdir -p $path; fi
+    echo $path
+}
+
+buildpath=$(create_dir $1)       
+installpath=$(create_dir $2)
+
+yum install -y m4 environment-modules systemd-devel
+source /etc/profile
+module purge
+
+set -e
+dep_dir="dependency"
+
+bash $dep_dir/bisheng-compiler-2.1.0.sh $buildpath $installpath
+module use $installpath/bisheng-compiler-2.1.0
+module load $installpath/bisheng-compiler-2.1.0/bisheng_modulefiles
+
+
+bash $dep_dir/blat-35.1.sh $buildpath $installpath
+module use $installpath/blat-35.1
+module load $installpath/blat-35.1/blat_modulefiles
+
+
+bash $dep_dir/bwa-0.7.17.sh $buildpath $installpath 
+module use $installpath/bwa-0.7.17
+module load  $installpath/bwa-0.7.17/bwa_modulefiles
+
+bash $dep_dir/samtools-1.15.1.sh $buildpath $installpath 
+module use  $installpath/samtools-1.15.1
+module load  $installpath/samtools-1.15.1/samtools_modulefiles  
+
+bash $dep_dir/bisheng-jdk1.8.0.sh $buildpath $installpath 
+module use $installpath/bisheng-jdk1.8.0_322
+module load  $installpath/bisheng-jdk1.8.0_322/jdk_modulefiles
+
+bash gatk-4.0.0.0.sh $buildpath $installpath
+
+mkdir -p pkg/gatk-4.0.0.0-hpc && cp -r $installpath/* pkg/gatk-4.0.0.0-hpc/
+cat>"pkg/gatk-4.0.0.0-hpc/run.sh"<<EOF
+#!/bin/bash
+sudo yum install -y wget tar environment-modules libatomic xz-devel ncurses-devel bzip2-devel curl-devel libpng-devel zlib-devel
+source /etc/profile &> /dev/null 
+current_dir="\$(cd "\$(dirname "\${BASH_SOURCE[0]}")" && pwd -P)"
+for f in "\${current_dir}"/*; do
+  if [[ -d "\${f}" && -f "\$(ls "\${f}"/*modulefiles 2>&1)" ]]; then
+    module use "\${f}" && module load "\${f}"/*modulefiles
+  fi
+done
+echo -e "\033[1;32;1mgatk environment initialization completed.\033[0m"
+EOF
+
+
+cd pkg/ && tar zcvf gatk-4.0.0.0-hpc.tar.gz gatk-4.0.0.0-hpc
+rm -rf $buildpath $installpath
diff --git a/LifeSciences/gatk-4.0.0.0/dependency/bisheng-compiler-2.1.0.sh b/LifeSciences/gatk-4.0.0.0/dependency/bisheng-compiler-2.1.0.sh
new file mode 100644
index 0000000000000000000000000000000000000000..a9f267c24cff4c3d2b8084cdc19684cfc8b0ec0b
--- /dev/null
+++ b/LifeSciences/gatk-4.0.0.0/dependency/bisheng-compiler-2.1.0.sh
@@ -0,0 +1,42 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash bisheng-compiler-2.1.0.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+[[ "$buildpath" == "$installpath" ]] && echo "构建路径与安装路径需为不同路径" && exit 1
+set -e
+yum install -y wget tar environment-modules libatomic
+
+wget -t 10 -c -P $buildpath https://mirrors.huaweicloud.com/kunpeng/archive/compiler/bisheng_compiler/bisheng-compiler-2.1.0-aarch64-linux.tar.gz
+tar xf $buildpath/bisheng-compiler-2.1.0-aarch64-linux.tar.gz -C $buildpath && rm -rf $buildpath/bisheng-compiler-2.1.0-aarch64-linux.tar.gz
+mkdir -p $installpath/bisheng-compiler-2.1.0 && cp -r $buildpath/bisheng-compiler-2.1.0-aarch64-linux/* $installpath/bisheng-compiler-2.1.0/
+
+cat>"$installpath/bisheng-compiler-2.1.0/bisheng_modulefiles"<<EOF
+#%Module1.0
+conflict bisheng
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set BISHENG \$pwd
+setenv BISHENG \$BISHENG
+prepend-path PATH \$BISHENG/bin
+prepend-path LD_LIBRARY_PATH \$BISHENG/lib
+EOF
+
+rm -rf $buildpath/bisheng-compiler-2.1.0-aarch64-linux
diff --git a/LifeSciences/gatk-4.0.0.0/dependency/bisheng-jdk1.8.0.sh b/LifeSciences/gatk-4.0.0.0/dependency/bisheng-jdk1.8.0.sh
new file mode 100644
index 0000000000000000000000000000000000000000..a546768336847dae67f991af01d09fe15718e4a3
--- /dev/null
+++ b/LifeSciences/gatk-4.0.0.0/dependency/bisheng-jdk1.8.0.sh
@@ -0,0 +1,44 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash bisheng-jdk1.8.0.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+[[ "$buildpath" == "$installpath" ]] && echo "构建路径与安装路径需为不同路径" && exit 1
+set -e
+yum install -y wget tar environment-modules
+
+wget  -t 10 -c -P  $buildpath https://mirrors.huaweicloud.com/kunpeng/archive/compiler/bisheng_jdk/bisheng-jdk-8u322-linux-aarch64.tar.gz
+tar -zxvf $buildpath/bisheng-jdk-8u322-linux-aarch64.tar.gz -C $buildpath && rm -rf $buildpath/bisheng-jdk-8u322-linux-aarch64.tar.gz
+mkdir -p $installpath/bisheng-jdk1.8.0_322 && cp -r $buildpath/bisheng-jdk1.8.0_322/* $installpath/bisheng-jdk1.8.0_322/
+cat>$installpath/bisheng-jdk1.8.0_322/jdk_modulefiles<<EOF
+#%Module1.0
+conflict jdk
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set JAVA_HOME \$pwd
+set JRE_HOME \$JAVA_HOME/jre
+setenv JAVA_HOME \$JAVA_HOME
+setenv JRE_HOME \$JRE_HOME
+
+prepend-path PATH \$JAVA_HOME/bin:\JRE_HOME/bin
+prepend-path CLASS_PATH \$JAVA_HOME/lib/dt.jar:\$JAVA_HOME/lib/tools.jar:\$JRE_HOME/lib
+EOF
+source /etc/profile 
+rm -rf $buildpath/bisheng-jdk1.8.0_322
diff --git a/LifeSciences/gatk-4.0.0.0/dependency/blat-35.1.sh b/LifeSciences/gatk-4.0.0.0/dependency/blat-35.1.sh
new file mode 100644
index 0000000000000000000000000000000000000000..74bda299d742fea602a9b1433bf8d66012be66bb
--- /dev/null
+++ b/LifeSciences/gatk-4.0.0.0/dependency/blat-35.1.sh
@@ -0,0 +1,48 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash blat.35.1.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+[[ "$buildpath" == "$installpath" ]] && echo "构建路径与安装路径需为不同路径" && exit 1
+set -e
+yum install -y wget tar environment-modules libatomic libpng-devel 
+
+
+wget -t 10 -c -P "$buildpath" https://github.com/djhshih/blat/archive/refs/tags/v35.1.tar.gz
+tar xf "$buildpath"/v35.1.tar.gz -C "$buildpath" && rm -rf "$buildpath"/v35.1.tar.gz
+
+mkdir -p "$installpath"/blat-35.1 && cp -r $buildpath/blat-35.1/* "$installpath"/blat-35.1
+cd "$installpath"/blat-35.1 && export MACHTYPE=aarch64
+sed -i "121c extern jmp_buf htmlRecover;" src/inc/htmshell.h
+
+DESTDIR="$installpath"/blat-35.1 make -j$(nproc) CC=clang
+cat>"$installpath/blat-35.1/blat_modulefiles"<<EOF 
+#%Module1.0
+conflict blat
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set BLAT \$pwd
+setenv BLAT \$BLAT
+prepend-path PATH \$BLAT/bin
+EOF
+
+
+rm -rf "$buildpath"/blat-35.1
+
diff --git a/LifeSciences/gatk-4.0.0.0/dependency/bwa-0.7.17.sh b/LifeSciences/gatk-4.0.0.0/dependency/bwa-0.7.17.sh
new file mode 100644
index 0000000000000000000000000000000000000000..c78856ad74b4d0c70cb4cd1f9bab9eae705d9f47
--- /dev/null
+++ b/LifeSciences/gatk-4.0.0.0/dependency/bwa-0.7.17.sh
@@ -0,0 +1,53 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash bwa-0.7.17.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+[[ "$buildpath" == "$installpath" ]] && echo "构建路径与安装路径需为不同路径" && exit 1
+set -e
+yum install -y wget tar environment-modules libatomic zlib-devel 
+
+cd "$buildpath"
+wget -t 20 -c https://github.com/lh3/bwa/archive/refs/tags/v0.7.17.tar.gz
+wget -t 20 -c https://github.com/DLTcollab/sse2neon/archive/refs/heads/master.zip
+tar xf v0.7.17.tar.gz && rm -rf v0.7.17.tar.gz
+unzip master.zip && rm -rf master.zip
+cp sse2neon-master/sse2neon.h bwa-0.7.17
+
+cd bwa-0.7.17
+sed -i "s/gcc/clang/" Makefile
+sed -i "29c #include \"sse2neon.h\"" ksw.c
+sed -i "33c extern const uint8_t rle_auxtab[8];" rle.h
+
+make -j$(nproc)
+mkdir -p "$installpath"/bwa-0.7.17
+cp -a ./* "$installpath"/bwa-0.7.17
+
+cat>"$installpath/bwa-0.7.17/bwa_modulefiles"<<EOF
+#%Module1.0
+conflict bwa
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set BWA \$pwd
+setenv BWA \$BWA
+prepend-path PATH \$BWA
+EOF
+
+rm -rf "$buildpath"/bwa-0.7.17
diff --git a/LifeSciences/gatk-4.0.0.0/dependency/samtools-1.15.1.sh b/LifeSciences/gatk-4.0.0.0/dependency/samtools-1.15.1.sh
new file mode 100644
index 0000000000000000000000000000000000000000..5da32ce13a959b183fc63dd4ba587664f4f70455
--- /dev/null
+++ b/LifeSciences/gatk-4.0.0.0/dependency/samtools-1.15.1.sh
@@ -0,0 +1,46 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash samtools-1.15.1.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+[[ "$buildpath" == "$installpath" ]] && echo "构建路径与安装路径需为不同路径" && exit 1
+set -e
+yum install xz-devel ncurses-devel bzip2-devel curl-devel -y 
+
+wget -t 20 -c -P "$buildpath" https://github.com/samtools/samtools/releases/download/1.15.1/samtools-1.15.1.tar.bz2
+tar xf "$buildpath"/samtools-1.15.1.tar.bz2 -C "$buildpath" && rm -rf "$buildpath"/samtools-1.15.1.tar.bz2
+cd "$buildpath"/samtools-1.15.1
+
+./configure --prefix="$installpath"/samtools-1.15.1 CC=clang
+make -j$(nproc)
+make install 
+
+cat>"$installpath/samtools-1.15.1/samtools_modulefiles"<<EOF
+#%Module1.0
+conflict samtools
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set SAMTOOLS \$pwd
+setenv SAMTOOLS \$SAMTOOLS
+prepend-path PATH \$SAMTOOLS/bin
+EOF
+
+rm -rf "$buildpath"/samtools-1.15.1
+
diff --git "a/LifeSciences/gatk-4.0.0.0/doc/GATK-4.0.0.0\345\272\224\347\224\250\346\265\213\350\257\225\346\212\245\345\221\212.docx" "b/LifeSciences/gatk-4.0.0.0/doc/GATK-4.0.0.0\345\272\224\347\224\250\346\265\213\350\257\225\346\212\245\345\221\212.docx"
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Binary files /dev/null and "b/LifeSciences/gatk-4.0.0.0/doc/GATK-4.0.0.0\345\272\224\347\224\250\346\265\213\350\257\225\346\212\245\345\221\212.docx" differ
diff --git "a/LifeSciences/gatk-4.0.0.0/doc/GATK-4.0.0.0\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx" "b/LifeSciences/gatk-4.0.0.0/doc/GATK-4.0.0.0\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx"
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Binary files /dev/null and "b/LifeSciences/gatk-4.0.0.0/doc/GATK-4.0.0.0\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx" differ
diff --git "a/LifeSciences/gatk-4.0.0.0/doc/\351\262\262\351\271\217\345\237\272\345\272\247\350\275\257\344\273\266\346\220\255\345\273\272\346\214\207\345\215\227.docx" "b/LifeSciences/gatk-4.0.0.0/doc/\351\262\262\351\271\217\345\237\272\345\272\247\350\275\257\344\273\266\346\220\255\345\273\272\346\214\207\345\215\227.docx"
new file mode 100644
index 0000000000000000000000000000000000000000..18bb02ef7e2ce33db1cd05cf8b524969d39f7af3
Binary files /dev/null and "b/LifeSciences/gatk-4.0.0.0/doc/\351\262\262\351\271\217\345\237\272\345\272\247\350\275\257\344\273\266\346\220\255\345\273\272\346\214\207\345\215\227.docx" differ
diff --git a/LifeSciences/gatk-4.0.0.0/gatk-4.0.0.0.sh b/LifeSciences/gatk-4.0.0.0/gatk-4.0.0.0.sh
new file mode 100644
index 0000000000000000000000000000000000000000..a09223aa028458d3b9237df848c03d00798eb80a
--- /dev/null
+++ b/LifeSciences/gatk-4.0.0.0/gatk-4.0.0.0.sh
@@ -0,0 +1,42 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash zlib-1.12.11.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+[[ "$buildpath" == "$installpath" ]] && echo "构建路径与安装路径需为不同路径" && exit 1
+set -e
+yum install -y wget tar environment-modules
+
+wget -t 10 -c -P $buildpath  https://github.com/broadinstitute/gatk/releases/download/4.0.0.0/gatk-4.0.0.0.zip
+cd $buildpath
+unzip  gatk-4.0.0.0.zip && rm -rf $buildpath/gatk-4.0.0.zip
+mkdir -p $installpath/gatk-4.0.0.0 && cp -r $buildpath/gatk-4.0.0.0/* $installpath/gatk-4.0.0.0
+
+cat>"$installpath/gatk-4.0.0.0/gatk_modulefiles"<<EOF
+#%Module1.0
+conflict gatk
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set GATK \$pwd
+setenv GATK \$GATK
+prepend-path PATH \$GATK
+EOF
+
+rm -rf $buildpath/gatk-4.0.0.0