diff --git a/LifeSciences/README.md b/LifeSciences/README.md
index d3aadd7a731e34a52f0676e2d2dc33797db13888..367845be4aa4e9181ab63cd49a1aa6d9bf688499 100644
--- a/LifeSciences/README.md
+++ b/LifeSciences/README.md
@@ -1,2 +1,3 @@
 Life Sciences Software List:
 - repositories: xxx
+- repositories: TRF-4.09.1
diff --git a/LifeSciences/TRF-4.09.1/TRF-4.09.1.sh b/LifeSciences/TRF-4.09.1/TRF-4.09.1.sh
new file mode 100644
index 0000000000000000000000000000000000000000..cff0be28b7c3ddc29962d1b2844a58df55817741
--- /dev/null
+++ b/LifeSciences/TRF-4.09.1/TRF-4.09.1.sh
@@ -0,0 +1,36 @@
+#!/bin/bash
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash TRF-4.09.1.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+if [[ "$1" == "$2" ]]; then echo "构建路径与安装路径不能相同，请输入正确的路径"; exit 1; fi
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+yum install -y  environment-modules csh time patch libatomic perl-Data-Dumper autoconf automake libtool numactl binutils systemd-devel valgrind flex wget
+
+set -e
+wget -t 0 -c -P $buildpath https://github.com/Benson-Genomics-Lab/TRF/archive/v4.09.1.tar.gz
+tar -xvf $buildpath/v4.09.1.tar.gz -C $buildpath && rm -rf $buildpath/v4.09.1.tar.gz
+
+cd $buildpath/TRF-4.09.1
+
+echo "make start ++++++++++++++++++++++++++++++++++++++++++++++++++++++"
+mkdir -p $installpath/TRF-4.09.1
+CC=clang ./configure --prefix=$installpath/TRF-4.09.1
+
+make
+make install
+echo "install end ++++++++++++++++++++++++++++++++++++++++++++++++++++++"
+echo $buildpath
+echo $installpath
+cp -r $buildpath/TRF-4.09.1/test_seqs.fasta $installpath/TRF-4.09.1/bin/
+rm -rf $buildpath/TRF-4.09.1
\ No newline at end of file
diff --git a/LifeSciences/TRF-4.09.1/build.sh b/LifeSciences/TRF-4.09.1/build.sh
new file mode 100644
index 0000000000000000000000000000000000000000..3f586a703dc5dde0f685c10016cd7de0055e5f83
--- /dev/null
+++ b/LifeSciences/TRF-4.09.1/build.sh
@@ -0,0 +1,53 @@
+#!/bin/bash
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash build.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+if [[ "$1" == "$2" ]]; then echo "构建路径与安装路径不能相同，请输入正确的路径"; exit 1; fi
+
+create_dir()
+{
+    local path=$1
+    [[ "$path" =~ .*/$ ]] && path=${path%/*}
+    if [[ ! -d "$path" && ! -f "$path" ]]; then mkdir -p $path; else path=$path`date "+%y%m%d%H%M%S"` && mkdir -p $path; fi
+    echo $path
+}
+
+buildpath=$(create_dir $1)
+installpath=$(create_dir $2)
+
+yum install -y m4 environment-modules systemd-devel
+source /etc/profile
+module purge
+
+set -e
+dep_dir="dependency"
+bash $dep_dir/bisheng-compiler-2.1.0.sh $buildpath $installpath
+module use $installpath/bisheng-compiler-2.1.0
+module load $installpath/bisheng-compiler-2.1.0/bisheng_modulefiles
+
+
+bash TRF-4.09.1.sh $buildpath $installpath
+
+mkdir -p pkg/TRF-4.09.1-hpc && cp -r $installpath/* pkg/TRF-4.09.1-hpc/
+
+cat>"pkg/TRF-4.09.1-hpc/run.sh"<<EOF
+#!/bin/bash
+yum install -y --nogpgcheck m4 environment-modules systemd-devel csh time patch libatomic perl-Data-Dumper \
+autoconf automake libtool numactl binutils valgrind flex wget tar libatomic gcc-c++ git
+rm -rf /usr/local/bin/trf
+CURRENT_DIR="\$(\$(cd "\$(dirname "\${BASH_SOURCE[0]}")") && pwd)"
+ln -s \${CURRENT_DIR}/TRF-4.09.1/bin/trf /usr/local/bin/trf
+EOF
+
+
+rm -rf pkg/TRF-4.09.1-hpc/bisheng-compiler-2.1.0
+cd pkg/ && tar zcvf TRF-4.09.1-hpc.tar.gz TRF-4.09.1-hpc
+
+rm -rf $buildpath $installpath
\ No newline at end of file
diff --git a/LifeSciences/TRF-4.09.1/dependency/bisheng-compiler-2.1.0.sh b/LifeSciences/TRF-4.09.1/dependency/bisheng-compiler-2.1.0.sh
new file mode 100644
index 0000000000000000000000000000000000000000..d0de4c777d481efdb2e96ca00a862b9db3b79243
--- /dev/null
+++ b/LifeSciences/TRF-4.09.1/dependency/bisheng-compiler-2.1.0.sh
@@ -0,0 +1,42 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash bisheng-compiler-2.1.0.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+if [[ "$1" == "$2" ]]; then echo "构建路径与安装路径不能相同，请输入正确的路径"; exit 1; fi
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+set -e
+yum install -y wget tar environment-modules libatomic
+
+wget -t 0 -c -P $buildpath https://mirrors.huaweicloud.com/kunpeng/archive/compiler/bisheng_compiler/bisheng-compiler-2.1.0-aarch64-linux.tar.gz
+tar xf $buildpath/bisheng-compiler-2.1.0-aarch64-linux.tar.gz -C $buildpath && rm -rf $buildpath/bisheng-compiler-2.1.0-aarch64-linux.tar.gz
+mkdir -p $installpath/bisheng-compiler-2.1.0 && cp -r $buildpath/bisheng-compiler-2.1.0-aarch64-linux/* $installpath/bisheng-compiler-2.1.0/
+
+cat>"$installpath/bisheng-compiler-2.1.0/bisheng_modulefiles"<<EOF
+#%Module1.0
+conflict bisheng
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set BISHENG \$pwd
+setenv BISHENG \$BISHENG
+prepend-path PATH \$BISHENG/bin
+prepend-path LD_LIBRARY_PATH \$BISHENG/lib
+EOF
+
+rm -rf $buildpath/bisheng-compiler-2.1.0-aarch64-linux
diff --git "a/LifeSciences/TRF-4.09.1/doc/TRF-4.09.1\345\272\224\347\224\250\346\265\213\350\257\225\346\212\245\345\221\212.docx" "b/LifeSciences/TRF-4.09.1/doc/TRF-4.09.1\345\272\224\347\224\250\346\265\213\350\257\225\346\212\245\345\221\212.docx"
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diff --git a/README.md b/README.md
index 85f84fb3c562de5f1faca4db311e82c79962df5a..ca3b545a4351604a64ad3548fb7518996dffa439 100644
--- a/README.md
+++ b/README.md
@@ -1,2 +1,3 @@
 HPC Software List:
 - repositories: MeteorologicalOcean/wrf-4.2
+- repositories: LifeSciences/TRF-4.09.1