diff --git a/LifeSciences/TRF-4.09.1/TRF-4.09.1.sh b/LifeSciences/TRF-4.09.1/TRF-4.09.1.sh
index cff0be28b7c3ddc29962d1b2844a58df55817741..2ac905e0ccb629414baf3921d917f066b977996a 100644
--- a/LifeSciences/TRF-4.09.1/TRF-4.09.1.sh
+++ b/LifeSciences/TRF-4.09.1/TRF-4.09.1.sh
@@ -33,4 +33,4 @@ echo "install end ++++++++++++++++++++++++++++++++++++++++++++++++++++++"
 echo $buildpath
 echo $installpath
 cp -r $buildpath/TRF-4.09.1/test_seqs.fasta $installpath/TRF-4.09.1/bin/
-rm -rf $buildpath/TRF-4.09.1
\ No newline at end of file
+rm -rf $buildpath/TRF-4.09.1
diff --git a/LifeSciences/TRF-4.09.1/build.sh b/LifeSciences/TRF-4.09.1/build.sh
index 3f586a703dc5dde0f685c10016cd7de0055e5f83..0d62f17c6813e6145fd8d475c60663ff14531919 100644
--- a/LifeSciences/TRF-4.09.1/build.sh
+++ b/LifeSciences/TRF-4.09.1/build.sh
@@ -9,7 +9,6 @@ fi
 
 [[ ! "$1" =~ ^/.* || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
 [[ ! "$2" =~ ^/.* || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
-if [[ "$1" == "$2" ]]; then echo "构建路径与安装路径不能相同，请输入正确的路径"; exit 1; fi
 
 create_dir()
 {
@@ -50,4 +49,4 @@ EOF
 rm -rf pkg/TRF-4.09.1-hpc/bisheng-compiler-2.1.0
 cd pkg/ && tar zcvf TRF-4.09.1-hpc.tar.gz TRF-4.09.1-hpc
 
-rm -rf $buildpath $installpath
\ No newline at end of file
+rm -rf $buildpath $installpath
diff --git "a/LifeSciences/TRF-4.09.1/doc/TRF-4.09.1\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx" "b/LifeSciences/TRF-4.09.1/doc/TRF-4.09.1\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx"
index b72121f2b2660ec826e9b8c9801d8d3c7a09c374..6f929d166a4894937d7a7ac236278c21e21c3de7 100644
Binary files "a/LifeSciences/TRF-4.09.1/doc/TRF-4.09.1\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx" and "b/LifeSciences/TRF-4.09.1/doc/TRF-4.09.1\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx" differ