diff --git a/LifeSciences/3d-dna-180922/3d-dna-180922.sh b/LifeSciences/3d-dna-180922/3d-dna-180922.sh
new file mode 100644
index 0000000000000000000000000000000000000000..1fdfdfb01236768a7c286fb0f2e007f8a756fe81
--- /dev/null
+++ b/LifeSciences/3d-dna-180922/3d-dna-180922.sh
@@ -0,0 +1,41 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "./3d-dna.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+if [[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]]; then echo "请输入正确的构建路径"; exit 1; fi
+if [[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]]; then echo "请输入正确的安装路径"; exit 1; fi
+if [[ "$1" == "$2" ]]; then echo "构建路径与安装路径不能相同，请输入正确的路径"; exit 1; fi
+
+buildpath=$1
+installpath=$2
+yum install -y wget tar environment-modules
+#安装3d-dna运行时环境 jdk
+yum install -y java-1.8.0-openjdk python3 python3-matplotlib python3-numpy python3-scipy
+#获取3d-dna源码，解压，增加执行权限
+wget -t 10 -c -P $buildpath https://codeload.github.com/aidenlab/3d-dna/zip/refs/heads/master
+cd $buildpath
+unzip master
+chmod +x -R $buildpath/3d-dna-master
+mkdir $installpath/3d-dna
+cp -r $buildpath/3d-dna-master/* $installpath/3d-dna/
+cat>"$installpath/3d-dna/3d-dna_modulefiles"<<EOF
+#%Module1.0
+conflict 3d-dna
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set dna \$pwd
+setenv dna \$dna
+prepend-path PATH \$dna
+EOF
+rm -rf $buildpath
diff --git a/LifeSciences/3d-dna-180922/build.sh b/LifeSciences/3d-dna-180922/build.sh
new file mode 100644
index 0000000000000000000000000000000000000000..ae0e556ce409bdb10dc64aa09d79cc10083a95f5
--- /dev/null
+++ b/LifeSciences/3d-dna-180922/build.sh
@@ -0,0 +1,51 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "./build.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+if [[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]]; then echo "请输入正确的构建路径"; exit 1; fi
+if [[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]]; then echo "请输入正确的安装路径"; exit 1; fi
+
+create_dir()
+{
+    local path=$1
+    [[ "$path" =~ .*/$ ]] && path=${path%/*}
+    if [[ ! -d "$path" && ! -f "$path" ]]; then mkdir -p $path; else path=$path`date "+%y%m%d%H%M%S"` && mkdir -p $path; fi
+    echo $path
+}
+
+buildpath=$(create_dir $1)
+installpath=$(create_dir $2)
+
+yum install -y wget tar environment-modules
+source /etc/profile
+module purge
+
+set -e
+dep_dir="dependency"
+#lastz安装
+bash $dep_dir/lastz.sh $buildpath $installpath
+module use $installpath/lastz-distrib
+module load $installpath/lastz-distrib/lastz_modulefiles
+#3d-dna安装
+bash 3d-dna-180922.sh $buildpath $installpath
+module use $installpath/3d-dna
+module load $installpath/3d-dna/3d-dna_modulefiles
+
+mkdir -p pkg/3d-dna-180922-hpc && cp -r $installpath/* pkg/3d-dna-180922-hpc/
+cat>"pkg/3d-dna-180922-hpc/run.sh"<<EOF
+yum install -y wget tar environment-modules java-1.8.0-openjdk python3 python3-matplotlib python3-numpy python3-scipy
+current_dir=\$(cd "\$(dirname "\${BASH_SOURCE[0]}")" &> /dev/null && pwd)
+for d in \$(ls \$current_dir)
+do
+    [ ! -f "\$current_dir/\$d" ] && cd \$current_dir/\$d && module use \$(pwd) && module load \$(pwd)/*modulefiles
+done
+EOF
+cd pkg/ && tar zcvf 3d-dna-180922-hpc.tar.gz 3d-dna-180922-hpc
+
+rm -rf $buildpath $installpath
diff --git a/LifeSciences/3d-dna-180922/dependency/lastz.sh b/LifeSciences/3d-dna-180922/dependency/lastz.sh
new file mode 100644
index 0000000000000000000000000000000000000000..1bb24223b6d2501b95fc63659616c200df9ef6a6
--- /dev/null
+++ b/LifeSciences/3d-dna-180922/dependency/lastz.sh
@@ -0,0 +1,42 @@
+#!/bin/bash
+
+if [[ $# -ne 2 ]]; then
+    echo "The format is as follows:"
+    echo "bash lastz.sh buildpath installpath"
+    echo ":param buildpath: 应用构建绝对路径"
+    echo ":param installpath: 应用安装绝对路径"
+    exit 1
+fi
+
+[[ ! "$1" =~ ^/.* || ! -d "$1" || "$1" = "/" ]] &&  echo "请输入正确的构建路径" && exit 1
+[[ ! "$2" =~ ^/.* || ! -d "$2" || "$2" = "/" ]] &&  echo "请输入正确的安装路径" && exit 1
+
+buildpath=$1
+installpath=$2
+[[ "$buildpath" =~ .*/$ ]] && buildpath=${buildpath%/*}
+[[ "$installpath" =~ .*/$ ]] && installpath=${installpath%/*}
+[[ "$buildpath" == "$installpath" ]] && echo "构建路径与安装路径需为不同路径" && exit 1
+set -e
+yum install -y wget tar environment-modules
+
+wget -t 10 -c -P $buildpath https://github.com/lastz/lastz/archive/refs/tags/1.04.15.tar.gz
+tar xf $buildpath/1.04.15.tar.gz -C $buildpath && rm -rf $buildpath/1.04.15.tar.gz
+cd $buildpath/lastz-1.04.15
+export LASTZ_INSTALL=$installpath/lastz-distrib/bin
+make -j$(nproc)
+make install
+cat>"$installpath/lastz-distrib/lastz_modulefiles"<<EOF
+#%Module1.0
+conflict lastz
+variable modfile [file normalize [info script]]
+proc getModulefileDir {} {
+    variable modfile
+    set modfile_path [file dirname \$modfile]
+    return \$modfile_path
+}
+set pwd [getModulefileDir]
+set lastz \$pwd
+setenv lastz \$lastz
+prepend-path PATH \$lastz/bin
+EOF
+rm -rf $buildpath/lastz-1.04.15
diff --git "a/LifeSciences/3d-dna-180922/doc/3d-dna-180922\345\272\224\347\224\250\346\265\213\350\257\225\346\212\245\345\221\212.docx" "b/LifeSciences/3d-dna-180922/doc/3d-dna-180922\345\272\224\347\224\250\346\265\213\350\257\225\346\212\245\345\221\212.docx"
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diff --git "a/LifeSciences/3d-dna-180922/doc/3d-dna-180922\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx" "b/LifeSciences/3d-dna-180922/doc/3d-dna-180922\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx"
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Binary files /dev/null and "b/LifeSciences/3d-dna-180922/doc/3d-dna-180922\345\272\224\347\224\250\347\247\273\346\244\215\346\214\207\345\215\227.docx" differ
diff --git a/LifeSciences/README.md b/LifeSciences/README.md
index 3be3521b50e9d7cae92fe80b0372874a8b5549df..be58fcda9c55c61ba9f6cf4d2e006a8b0ba5e83c 100644
--- a/LifeSciences/README.md
+++ b/LifeSciences/README.md
@@ -5,3 +5,10 @@ Life Sciences Software List:
 - repositories: trimmomatic-0.40
 - repositories: RepeatMasker-4.1.2
 - repositories: eigensoft-7.2.1
+- repositories: redundans-v0.13c
+- repositories: bamtools-2.5.2
+- repositories: flye-2.9
+- repositories: Manta-1.6.0
+- repositories: hmmer-3.3.2
+- repositories: Muscle-5.1
+- repositories: 3d-dna-180922
diff --git a/README.md b/README.md
index 3ae16b3b0c04e199da1a5fba71de6c8f12437eda..e0f6b168101fc54e4b69d1bf147172fdd5d45aad 100644
--- a/README.md
+++ b/README.md
@@ -5,3 +5,12 @@ HPC Software List:
 - repositories: LifeSciences/trimmomatic-0.40
 - repositories: LifeSciences/RepeatMasker-4.1.2
 - repositories: LifeSciences/eigensoft-7.2.1
+- repositories: LifeSciences/redundans-v0.13c
+- repositories: LifeSciences/bamtools-2.5.2
+- repositories: LifeSciences/flye-2.9
+- repositories: LifeSciences/Manta-1.6.0
+- repositories: ManufacturingSimulation/OpenLB-1.5-0
+- repositories: LifeSciences/hmmer-3.3.2
+- repositories: ManufacturingSimulation/SU2-6.0.1
+- repositories: LifeSciences/Muscle-5.1
+- repositories: LifeSciences/3d-dna-180922